The kit is suitable for real-time RT-PCR (RT-qPCR) that contains a gDNA Remover which can effectively eliminate the contamination of genomic DNA (gDNA) or other double stranded DNA during the analysis of gene expression. To accurately analyze gene expression, it is necessary to detect cDNA in samples without contaminating DNA. To avoid amplification of gDNA, primers can be designed on different exons spanning introns. However, there may be cases where a suitable primer cannot be designed, as with a gene with a single exon or a gene without a long intron. Also, it may be difficult to avoid unexpected amplification from gDNA due to non-specific amplification or the existence of pseudo-genes. Moreover, some labs are heavily contaminated by the PCR products of previous tests. This kit offers a potent gDNA Remover that can eliminate double stranded DNA in RNA sample in 5 minutes at room temperature without loss of RNA. Then the first strand of cDNA is synthesized by adding the 4× EZRT Mix II. Reaction products are applicable to subsequent PCR, qPCR.